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Singleron Biotechnologies microfluidic devices
Microfluidic Devices, supplied by Singleron Biotechnologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microfluidic+devices/chip+microfluidic/pm42234562-306-6-34
Average 86 stars, based on 1 article reviews
microfluidic devices - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Single Cell:

Article Title: Single-cell transcriptome profiling implicates the psychological stress-induced disruption of spermatogenesis
Article Snippet: Single-cell suspensions were prepared at a concentration of 1 × 10 5 cells/mL in PBS (HyClone). .. Single-cell suspensions were then loaded onto microfluidic devices and scRNA-seq libraries were constructed according to Singleron GEXSCOPE protocol by GEXSCOPE Single-Cell RNA Library Kit (Singleron Biotechnologies). ..

Article Title: BET inhibitor in combination with BCG vaccine enhances antitumor efficacy and orchestrates T cell reprogramming for melanoma.
Article Snippet: Single cell suspensions (13 105 cells/mL) with PBS (HyClone. .. Logan UT, USA) were loaded into microfluidic devices using the Singleron Matrix Single Cell Processing System (Singleron Biotechnologies, Koln, Germany). ..

Article Title: Endothelial PDLIM5 promotes tip cell filopodia formation and tumor angiogenesis by regulating ACTN1/ACTN4-dependent actin bundling
Article Snippet: .. Single-cell suspensions were prepared at a concentration of 1 × 10 5 cells/mL in PBS and loaded onto microfluidic devices. scRNA-seq libraries were constructed using the GEXSCOPE® Single-Cell RNA Library Kit (Singleron Biotechnologies, China). ..

Article Title: Landscape of the Peripheral Immune Response Induced by Intraoperative Radiotherapy Combined with Surgery in Early Breast Cancer Patients.
Article Snippet: Single-Cell RNA Sequencing: Cellular viability of all freshly isolated PBMCs exceeded 90%, as assessed microscopically using trypan blue (Sigma, USA). .. Single-cell suspensions in PBS were loaded into microfluidic devices using the Singleron Matrix Single Cell Processing System from Singleron Biotechnologies in China. .. Subsequently, single-cell RNA sequencing (scRNA-seq) libraries were constructed following the GEXSCOPE Single Cell RNA Library Kits protocol, also from Singleron Biotechnologies, China.

Article Title: LZTR1 regulates epithelial MHC-I expression via NF-κB1 to modulate CD8 + T cells activation.
Article Snippet: For human (epidermis) and mouse (epidermis & dermis) total single-cell suspension samples without sorting, we used Dead Cell Removal Kit (cat. 130-090-101, Miltenyi Biotec) to improve cell viability to meet the requirements of scRNA-seq. .. Single-cell suspensions (1 × 105 cells/mL) with PBS were loaded into microfluidic devices using the Singleron Matrix® Single Cell Processing System (Singleron Biotechnologies, Koln, Germany). ..

Article Title: ILC2-derived CGRP triggers acute inflammation and nociceptive responses in bacterial cystitis.
Article Snippet: Il33 / and Calcaf/f mice were obtained from GemPharmatech Co., Ltd. Lyz2cre and Ramp1f/f mice were purchased from Shanghai Model Organisms Center, Inc. All mice were housed in the specific pathogen-free animal facility of our institution. .. Single-cell suspensions were loaded onto microfluidic devices for scRNA-seq library construction following the Singleron GEXSCOPE protocol in the GEXSCOPE Single-Cell RNA Library Kit (Singleron Biotechnologies). .. Libraries were sequenced on Illumina NovaSeq6000 with 150 bp paired-end reads after pooling individual libraries at 4 nM concentration.

Article Title: CD168 + macrophages promote hepatocellular carcinoma tumor stemness and progression through TOP2A /β-catenin/ YAP1 axis
Article Snippet: The concentration of single-cell suspension was adjusted to 1 × 10 5 cells/mL in PBS (HyClone). .. Single-cell suspensions were then loaded onto microfluidic devices and scRNA-seq libraries were constructed according to Singleron GEXSCOPE® protocol by GEXSCOPE® Single-Cell RNA Library Kit (Singleron Biotechnologies). ..

Article Title: Spatial and temporal single cell multi-omics in mice reveals macrophage-SFRP4+ stroma interactions promoting endometrial regeneration via TNF-α.
Article Snippet: Single-cell RNA sequencing Single-cell suspensions with 1×105 cells/mL in PBS (HyClone) were prepared for sequencing. .. Single-cell suspensions were then loaded onto microfluidic devices, and scRNA-seq libraries were constructed according to the Singleron GEXSCOPE® protocol using 20 Cell Reports 45, 117434, June 23, 2026 the GEXSCOPE® Single-Cell RNA Library Kit (Singleron Biotechnologies). ..

Construct:

Article Title: Single-cell transcriptome profiling implicates the psychological stress-induced disruption of spermatogenesis
Article Snippet: Single-cell suspensions were prepared at a concentration of 1 × 10 5 cells/mL in PBS (HyClone). .. Single-cell suspensions were then loaded onto microfluidic devices and scRNA-seq libraries were constructed according to Singleron GEXSCOPE protocol by GEXSCOPE Single-Cell RNA Library Kit (Singleron Biotechnologies). ..

Article Title: Endothelial PDLIM5 promotes tip cell filopodia formation and tumor angiogenesis by regulating ACTN1/ACTN4-dependent actin bundling
Article Snippet: .. Single-cell suspensions were prepared at a concentration of 1 × 10 5 cells/mL in PBS and loaded onto microfluidic devices. scRNA-seq libraries were constructed using the GEXSCOPE® Single-Cell RNA Library Kit (Singleron Biotechnologies, China). ..

Article Title: CD168 + macrophages promote hepatocellular carcinoma tumor stemness and progression through TOP2A /β-catenin/ YAP1 axis
Article Snippet: The concentration of single-cell suspension was adjusted to 1 × 10 5 cells/mL in PBS (HyClone). .. Single-cell suspensions were then loaded onto microfluidic devices and scRNA-seq libraries were constructed according to Singleron GEXSCOPE® protocol by GEXSCOPE® Single-Cell RNA Library Kit (Singleron Biotechnologies). ..

Article Title: Spatial and temporal single cell multi-omics in mice reveals macrophage-SFRP4+ stroma interactions promoting endometrial regeneration via TNF-α.
Article Snippet: Single-cell RNA sequencing Single-cell suspensions with 1×105 cells/mL in PBS (HyClone) were prepared for sequencing. .. Single-cell suspensions were then loaded onto microfluidic devices, and scRNA-seq libraries were constructed according to the Singleron GEXSCOPE® protocol using 20 Cell Reports 45, 117434, June 23, 2026 the GEXSCOPE® Single-Cell RNA Library Kit (Singleron Biotechnologies). ..

Concentration Assay:

Article Title: Endothelial PDLIM5 promotes tip cell filopodia formation and tumor angiogenesis by regulating ACTN1/ACTN4-dependent actin bundling
Article Snippet: .. Single-cell suspensions were prepared at a concentration of 1 × 10 5 cells/mL in PBS and loaded onto microfluidic devices. scRNA-seq libraries were constructed using the GEXSCOPE® Single-Cell RNA Library Kit (Singleron Biotechnologies, China). ..



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Image Search Results


Microfluidics device design and fabrication. (A) Photograph of the microfluidics device. (B) Cartoon representation of the microfluidics device used in these studies. The general layout is shown with the 3 inlet and outlet ports labeled. The dual Herringbone structures for efficient solution mixing are also shown. (C) Device fabrication flowchart. The flowchart includes all steps for device construction as well as pretreatment for MS analysis.

Journal: ACS Omega

Article Title: Integrating Simple Microfluidics Design/Fabrication with a Novel Ionization Source for Time-Resolved Chemical Reaction Studies by Mass Spectrometry

doi: 10.1021/acsomega.5c12681

Figure Lengend Snippet: Microfluidics device design and fabrication. (A) Photograph of the microfluidics device. (B) Cartoon representation of the microfluidics device used in these studies. The general layout is shown with the 3 inlet and outlet ports labeled. The dual Herringbone structures for efficient solution mixing are also shown. (C) Device fabrication flowchart. The flowchart includes all steps for device construction as well as pretreatment for MS analysis.

Article Snippet: All three solutions were injected into the microfluidic device using two Fusion 6000 syringe pumps (Chemyx, Stafford, TX) before connecting to the cVSSI device via tubing.

Techniques: Labeling

Total deuterium uptake of the microfluidics setup employing different total flow rates. The PTFE tubing length associated with each flow rate is provided above the respective data point. The dashed line shows the weighted m / z value of unreacted myoglobin 14+ ions. Error bars represent one standard deviation about the mean for triplicate measurements.

Journal: ACS Omega

Article Title: Integrating Simple Microfluidics Design/Fabrication with a Novel Ionization Source for Time-Resolved Chemical Reaction Studies by Mass Spectrometry

doi: 10.1021/acsomega.5c12681

Figure Lengend Snippet: Total deuterium uptake of the microfluidics setup employing different total flow rates. The PTFE tubing length associated with each flow rate is provided above the respective data point. The dashed line shows the weighted m / z value of unreacted myoglobin 14+ ions. Error bars represent one standard deviation about the mean for triplicate measurements.

Article Snippet: All three solutions were injected into the microfluidic device using two Fusion 6000 syringe pumps (Chemyx, Stafford, TX) before connecting to the cVSSI device via tubing.

Techniques: Standard Deviation